ezh2 incubator unc 1999 Search Results


ezh2 inhibitor  (Tocris)
93
Tocris ezh2 inhibitor
( A ) H3K27me3 ChIP-qPCR in ESCs (Left) and EpiLCs (Middle) in WT and the ∆ mutant. There is no significant effect on polycomb dynamics in ESCs. In EpiLCs, the ∆ mutant retains residual H3K27me3 relative to WT. H3K27me3 ChIP-qPCR in WT and ∆ ESCs and EpiLCs at control loci are displayed in right panel. Data shown as ±s.e.m. from three biological replicates for each genotype. ( B ) Alleles generated by CRISPR/Cas9-mediated mutagenesis of Eed in WT and ∆ contexts. ( C ) Western blot confirming loss of EED protein and H3K27me3 in Eed-/- cell lines. ( D ) Western blot showing no detection of H3K27me3 loss after four days of incubation with PRC2 inhibitor. Negative control exhibited strong H3K27me3 signal. ( E ) RT-qPCR of Zdbf2 in absence of CTCF partition with <t>EZH2</t> inhibitor. In ∆CTCF_PS mutants, there is a further increase of Zdbf2 de-repression when H3K27me3 is depleted. Data shown as ±s.e.m. from three biological replicates for each genotype. Statistical analyses were performed by two-tailed unpaired t-test: *p≤0.05, **≤0.01, ***p≤0.001.
Ezh2 Inhibitor, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ezh2 incubator unc 1999  (Tocris)
91
Tocris ezh2 incubator unc 1999
( A ) H3K27me3 ChIP-qPCR in ESCs (Left) and EpiLCs (Middle) in WT and the ∆ mutant. There is no significant effect on polycomb dynamics in ESCs. In EpiLCs, the ∆ mutant retains residual H3K27me3 relative to WT. H3K27me3 ChIP-qPCR in WT and ∆ ESCs and EpiLCs at control loci are displayed in right panel. Data shown as ±s.e.m. from three biological replicates for each genotype. ( B ) Alleles generated by CRISPR/Cas9-mediated mutagenesis of Eed in WT and ∆ contexts. ( C ) Western blot confirming loss of EED protein and H3K27me3 in Eed-/- cell lines. ( D ) Western blot showing no detection of H3K27me3 loss after four days of incubation with PRC2 inhibitor. Negative control exhibited strong H3K27me3 signal. ( E ) RT-qPCR of Zdbf2 in absence of CTCF partition with <t>EZH2</t> inhibitor. In ∆CTCF_PS mutants, there is a further increase of Zdbf2 de-repression when H3K27me3 is depleted. Data shown as ±s.e.m. from three biological replicates for each genotype. Statistical analyses were performed by two-tailed unpaired t-test: *p≤0.05, **≤0.01, ***p≤0.001.
Ezh2 Incubator Unc 1999, supplied by Tocris, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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drug ezh2 inhibitor tocris bioscience unc 1999 chemical compound  (Tocris)
94
Tocris drug ezh2 inhibitor tocris bioscience unc 1999 chemical compound
( A ) H3K27me3 ChIP-qPCR in ESCs (Left) and EpiLCs (Middle) in WT and the ∆ mutant. There is no significant effect on polycomb dynamics in ESCs. In EpiLCs, the ∆ mutant retains residual H3K27me3 relative to WT. H3K27me3 ChIP-qPCR in WT and ∆ ESCs and EpiLCs at control loci are displayed in right panel. Data shown as ±s.e.m. from three biological replicates for each genotype. ( B ) Alleles generated by CRISPR/Cas9-mediated mutagenesis of Eed in WT and ∆ contexts. ( C ) Western blot confirming loss of EED protein and H3K27me3 in Eed-/- cell lines. ( D ) Western blot showing no detection of H3K27me3 loss after four days of incubation with PRC2 inhibitor. Negative control exhibited strong H3K27me3 signal. ( E ) RT-qPCR of Zdbf2 in absence of CTCF partition with <t>EZH2</t> inhibitor. In ∆CTCF_PS mutants, there is a further increase of Zdbf2 de-repression when H3K27me3 is depleted. Data shown as ±s.e.m. from three biological replicates for each genotype. Statistical analyses were performed by two-tailed unpaired t-test: *p≤0.05, **≤0.01, ***p≤0.001.
Drug Ezh2 Inhibitor Tocris Bioscience Unc 1999 Chemical Compound, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
drug ezh2 inhibitor tocris bioscience unc 1999 chemical compound - by Bioz Stars, 2026-05
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Image Search Results


( A ) H3K27me3 ChIP-qPCR in ESCs (Left) and EpiLCs (Middle) in WT and the ∆ mutant. There is no significant effect on polycomb dynamics in ESCs. In EpiLCs, the ∆ mutant retains residual H3K27me3 relative to WT. H3K27me3 ChIP-qPCR in WT and ∆ ESCs and EpiLCs at control loci are displayed in right panel. Data shown as ±s.e.m. from three biological replicates for each genotype. ( B ) Alleles generated by CRISPR/Cas9-mediated mutagenesis of Eed in WT and ∆ contexts. ( C ) Western blot confirming loss of EED protein and H3K27me3 in Eed-/- cell lines. ( D ) Western blot showing no detection of H3K27me3 loss after four days of incubation with PRC2 inhibitor. Negative control exhibited strong H3K27me3 signal. ( E ) RT-qPCR of Zdbf2 in absence of CTCF partition with EZH2 inhibitor. In ∆CTCF_PS mutants, there is a further increase of Zdbf2 de-repression when H3K27me3 is depleted. Data shown as ±s.e.m. from three biological replicates for each genotype. Statistical analyses were performed by two-tailed unpaired t-test: *p≤0.05, **≤0.01, ***p≤0.001.

Journal: eLife

Article Title: Dynamic enhancer partitioning instructs activation of a growth-related gene during exit from naïve pluripotency

doi: 10.7554/eLife.44057

Figure Lengend Snippet: ( A ) H3K27me3 ChIP-qPCR in ESCs (Left) and EpiLCs (Middle) in WT and the ∆ mutant. There is no significant effect on polycomb dynamics in ESCs. In EpiLCs, the ∆ mutant retains residual H3K27me3 relative to WT. H3K27me3 ChIP-qPCR in WT and ∆ ESCs and EpiLCs at control loci are displayed in right panel. Data shown as ±s.e.m. from three biological replicates for each genotype. ( B ) Alleles generated by CRISPR/Cas9-mediated mutagenesis of Eed in WT and ∆ contexts. ( C ) Western blot confirming loss of EED protein and H3K27me3 in Eed-/- cell lines. ( D ) Western blot showing no detection of H3K27me3 loss after four days of incubation with PRC2 inhibitor. Negative control exhibited strong H3K27me3 signal. ( E ) RT-qPCR of Zdbf2 in absence of CTCF partition with EZH2 inhibitor. In ∆CTCF_PS mutants, there is a further increase of Zdbf2 de-repression when H3K27me3 is depleted. Data shown as ±s.e.m. from three biological replicates for each genotype. Statistical analyses were performed by two-tailed unpaired t-test: *p≤0.05, **≤0.01, ***p≤0.001.

Article Snippet: Chemical Compound, Drug , EZH2 Inhibitor , Tocris Bioscience , UNC 1999 , .

Techniques: ChIP-qPCR, Mutagenesis, Control, Generated, CRISPR, Western Blot, Incubation, Negative Control, Quantitative RT-PCR, Two Tailed Test

Journal: eLife

Article Title: Dynamic enhancer partitioning instructs activation of a growth-related gene during exit from naïve pluripotency

doi: 10.7554/eLife.44057

Figure Lengend Snippet:

Article Snippet: Chemical Compound, Drug , EZH2 Inhibitor , Tocris Bioscience , UNC 1999 , .

Techniques: CRISPR, Generated, Mutagenesis, Negative Control, Recombinant, Software

( A ) H3K27me3 ChIP-qPCR in ESCs (Left) and EpiLCs (Middle) in WT and the ∆ mutant. There is no significant effect on polycomb dynamics in ESCs. In EpiLCs, the ∆ mutant retains residual H3K27me3 relative to WT. H3K27me3 ChIP-qPCR in WT and ∆ ESCs and EpiLCs at control loci are displayed in right panel. Data shown as ±s.e.m. from three biological replicates for each genotype. ( B ) Alleles generated by CRISPR/Cas9-mediated mutagenesis of Eed in WT and ∆ contexts. ( C ) Western blot confirming loss of EED protein and H3K27me3 in Eed-/- cell lines. ( D ) Western blot showing no detection of H3K27me3 loss after four days of incubation with PRC2 inhibitor. Negative control exhibited strong H3K27me3 signal. ( E ) RT-qPCR of Zdbf2 in absence of CTCF partition with EZH2 inhibitor. In ∆CTCF_PS mutants, there is a further increase of Zdbf2 de-repression when H3K27me3 is depleted. Data shown as ±s.e.m. from three biological replicates for each genotype. Statistical analyses were performed by two-tailed unpaired t-test: *p≤0.05, **≤0.01, ***p≤0.001.

Journal: eLife

Article Title: Dynamic enhancer partitioning instructs activation of a growth-related gene during exit from naïve pluripotency

doi: 10.7554/eLife.44057

Figure Lengend Snippet: ( A ) H3K27me3 ChIP-qPCR in ESCs (Left) and EpiLCs (Middle) in WT and the ∆ mutant. There is no significant effect on polycomb dynamics in ESCs. In EpiLCs, the ∆ mutant retains residual H3K27me3 relative to WT. H3K27me3 ChIP-qPCR in WT and ∆ ESCs and EpiLCs at control loci are displayed in right panel. Data shown as ±s.e.m. from three biological replicates for each genotype. ( B ) Alleles generated by CRISPR/Cas9-mediated mutagenesis of Eed in WT and ∆ contexts. ( C ) Western blot confirming loss of EED protein and H3K27me3 in Eed-/- cell lines. ( D ) Western blot showing no detection of H3K27me3 loss after four days of incubation with PRC2 inhibitor. Negative control exhibited strong H3K27me3 signal. ( E ) RT-qPCR of Zdbf2 in absence of CTCF partition with EZH2 inhibitor. In ∆CTCF_PS mutants, there is a further increase of Zdbf2 de-repression when H3K27me3 is depleted. Data shown as ±s.e.m. from three biological replicates for each genotype. Statistical analyses were performed by two-tailed unpaired t-test: *p≤0.05, **≤0.01, ***p≤0.001.

Article Snippet: For EZH2 inhibition experiments, the EZH2 incubator UNC 1999 (or its negative control UNC 2400, Tocris Bioscience) was added to media at a 1 μM final concentration for four days.

Techniques: ChIP-qPCR, Mutagenesis, Control, Generated, CRISPR, Western Blot, Incubation, Negative Control, Quantitative RT-PCR, Two Tailed Test

Journal: eLife

Article Title: Dynamic enhancer partitioning instructs activation of a growth-related gene during exit from naïve pluripotency

doi: 10.7554/eLife.44057

Figure Lengend Snippet:

Article Snippet: For EZH2 inhibition experiments, the EZH2 incubator UNC 1999 (or its negative control UNC 2400, Tocris Bioscience) was added to media at a 1 μM final concentration for four days.

Techniques: CRISPR, Generated, Mutagenesis, Negative Control, Recombinant, Software